Human VEGF ELISA Kit INTRODUCTION

VEGF （Vascular endothelial growth factor） is also called VEGF-A,
following the identification of several VEGF-related factors （VEGF-B,
VEGF-C, VEGF-D, VEGF-E）. VEGF significantly influence vascular
permeability and is a strong angiogenic protein in several bioassays and
probably also plays a role in neovascularisation under physiological
conditions. VEGF plays a role in the development and function of primate
follicles and the ovarian corpus luteum, supporting the proliferation of blood
vessels. The differentiation of adipocytes, of pheochromocytomas, and
myocytes is accompanied by the controlled expression of VEGF. It has been
demonstrated that inhibition of VEGF activity by treatment with a
monoclonal antibody specific for VEGF can suppress tumor growth in vivo.

Human VEGF ELISA （Enzyme-Linked Immunosorbent Assay） kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in cell lysate and tissue lysate. This assay employs an antibody specific for human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed,a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, Human VEGF ELISA Kit and the intensity of the color is measured at 450 nm.

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