 | 培安有限公司 |
會(huì)員.png)
010-65528800
高 （市場(chǎng)部）
- 電話：
- 010-65528800
- 手機(jī)：
- 售后電話：
- 010-64278205
- 傳真：
- 010-65519722
- 聯(lián)系我時(shí)，
- 告知來(lái)自化工儀器網(wǎng)
- 個(gè)性化：
- www.pynnco.net
- 商鋪網(wǎng)址：
- http://www.euroanunturi.com/st26894/
- 公司網(wǎng)站：
- http://www.pynnco.com
蛋白質(zhì)組學(xué)應(yīng)用：壓力循環(huán)技術(shù)催進(jìn)蛋白酶解能力
閱讀：2467發(fā)布時(shí)間：2009-5-20
蛋白質(zhì)組學(xué)應(yīng)用：壓力循環(huán)技術(shù)催進(jìn)蛋白酶解能力
Application of Pressurized Solvents for Ultrafast Trypsin Hydrolysis in Proteomics:
Proteomics on the Fly
摘要:
一種在高壓下迅速水解蛋白質(zhì)的新方法: 利用壓力循環(huán)技術(shù)范圍為5-35 kpsi;從而協(xié)助蛋白質(zhì)組學(xué)分析。壓力循環(huán)技術(shù)成功在60sec內(nèi)把單一蛋白質(zhì)及復(fù)雜的蛋白質(zhì)水解.然后利用反相液相色譜分析。本文中, 利用壓力循環(huán)技術(shù)新方法進(jìn)行Shewanella oneidensis 多肽水解與傳統(tǒng)的“過夜”樣品制備方法作對(duì)比. 新方法-壓力循環(huán)的優(yōu)勢(shì):包括簡(jiǎn)化樣品處理過程，易于實(shí)施，實(shí)驗(yàn)中樣品沒有交叉污染，和成本效率高。
Abstract:
A new method for rapid proteolytic digestion of proteins under high pressure that uses pressure cycling technology in the range of 5-35 kpsi was demonstrated for proteomic analysis. Successful in-solution digestions of single proteins and complex protein mixtures were achieved in 60 s and then analyzed by reversed phase liquid chromatography-electrospray ionization ion trap-mass spectrometry. Method performance in terms of the number of Shewanella oneidensis peptides and proteins identified in a shotgun approach was evaluated relative to a traditional “overnight” sample preparation method. Advantages of the new method include greatly simplified sample processing, easy implementation, no cross contamination among samples, and cost effectiveness.
更多信息，請(qǐng)咨詢，：sales@pynnco.com, 或?yàn)g覽我們的：www.pynnco.com
以上信息由企業(yè)自行提供，信息內(nèi)容的真實(shí)性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé)，化工儀器網(wǎng)對(duì)此不承擔(dān)任何保證責(zé)任。 溫馨提示：為規(guī)避購(gòu)買風(fēng)險(xiǎn)，建議您在購(gòu)買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。