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EnzyChrom α-Amylase Assay Kit 淀粉|酶測試盒

產(chǎn)品信息

產(chǎn)品說明    

淀粉|酶是一種水解酶，它能夠作用于淀粉分子中的a-1，4-糖苷鍵，將其分解為葡萄糖。a-淀粉|酶可以作用于直鏈淀粉和支鏈淀粉，切開淀粉鏈?zhǔn)蛊滢D(zhuǎn)化成麥芽糖、麥芽三糖、葡萄糖和極限糊精。對哺乳動物來說，a-淀粉|酶是主要的消化酶，其含量增高可能與涎腺損傷、腮腺炎、胰腺炎或腎衰竭等疾病有關(guān)。在科研和藥物開發(fā)中，簡單、自動化的a-淀粉\酶檢測手段是zui為理想的。本公司的的a-淀粉|酶檢測包含兩個步驟：(1) a-淀粉|酶將樣品中的淀粉水解，其產(chǎn)物在a-葡糖苷酶和葡糖氧化酶的作用下，轉(zhuǎn)變?yōu)槠咸烟呛碗p氧水。(2) 利用一種比色試劑，檢測出雙氧水濃度。

測定方法  比色法（585nm）定量測定α-淀粉|酶活性。

產(chǎn)品特點  

檢測敏感、準(zhǔn)確：用96孔板檢測，a-淀粉|酶活性檢測范圍為0.3 - 50 U/L。

操作簡便：整個檢測過程為：加入一種工作試劑并反應(yīng)15分鐘，然后加入檢測試劑再反應(yīng)20分鐘后，在585nm下讀取吸光度。

產(chǎn)品用途  直接檢測血液、唾液、尿液、谷物和其他農(nóng)產(chǎn)品中a-淀粉|酶的活性。

儲藏條件： 冰運。底物和檢測試劑在4°C的環(huán)境下保存，其它試劑在-20°C下保存。

Description    Amylase belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimay yielding maltotriose and maltose, glucose and "limit dextrin" from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure.Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. our EnzyChromTM a-amylase assay method involves two steps: (1). α-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by α-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen peroxidconcentration is determined with a colorimetric reagent.

Applications     Direct assays of α-amylase activity in blood, saliva, urine, grains and other agricultural samples.

Key features    Sensitive and accurate. Linear detection range 0.3 to 50 U/L α-amylase in 96-well plate assay.

Convenient. The procedure involves adding a single working reagent, incubation for 15 min, followed by the detection reagent and a 20-min incubation and reading the optical density at 585 nm.    

Storage conditions. Kit is shipped on dry ice. Store Substrate and Detection Reagent at 4°C and others at -20°C. Shelf life of at least 6 months (see expiry dates on labels).