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上海源葉生物科技有限公司
主營產(chǎn)品： S30260異硫氰酸胍,30259鹽酸胍,嗜熱菌蛋白酶 |
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15921386130
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- 何小姐
- 電話：
- 86-021-61559134
- 手機：
- 15921386130
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- 86-021-55068248
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- 上海市松江區(qū)長塔路465號6幢
- 郵編：
- 200433
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- www.shyuanye.com
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更新時間：2025-05-16 07:12:55瀏覽次數(shù)：145
聯(lián)系我們時請說明是化工儀器網(wǎng)上看到的信息，謝謝!
- 提示：詳情請下載說明書。
- 產(chǎn)品描述： Q-VD-OPh 是一種不可逆的泛胱天蛋白酶 (caspase) 抑制劑，具有高效的抗凋亡能力。抑制胱天蛋白酶 7 的 IC50 值別為 48 nM，抑制胱天蛋白酶 1，3，8，9，10，12 的 IC50 值在 25-400 nM 之間。Q-VD-OPh 可抑制 HIV 感染。Q-VD-OPh 能透過血腦屏障。
- 靶點： Caspase-1; Caspase-3; Caspase-8; Caspase-9
- 體外研究： Q-VD-OPh is a potent inhibitor of caspase-7 with an IC50 of 48 nM utilizing a cell-free assay consisting of human recombinant caspase-7, Q-VD-OPh, and the substrate AMC-DEVD-pNa. Q-VD-OPh fully inhibits caspase-3 and -7 activity at 0.05 μM. Caspase-8 is also inhibited at low Q-VD-OPh concentrations. The cleavage of PARP-1 is fully prevented at 10 μM Q-VD-OPh. DNA fragmentation and disruption of the cell membrane functionality are both prevented at 2 μM Q-VD-OPh. Q-VD-OPh is significantly more effective in preventing apoptosis than the widely used inhibitors, ZVAD-fmk and Boc-D-fmk, and is also equally effective in preventing apoptosis mediated by the three major apoptotic pathways, caspase 9/3, caspase 8/10, and caspase12. Q-VD-OPh is not toxic to cells even at extremely high concentrations. QVD is also able to increase the expression of differentiation markers in acute myeloid leukemia (AmL) blasts. QVD alone or combined with VDDs increases differentiation and HPK1-cJun signaling in AmL cell context-dependent manner.
- 體內(nèi)研究： Chronic treatment with Q-VD-OPh prevents caspase-7 activation and limits the pathological changes associated with tau, including caspase cleavage. Q-VD-OPh could be a potential therapeutic compound for the treatment of Alzheimer's disease.
- 細胞實驗： Caspase inhibitors are added at the indicated concentrations 30 minutes prior to the addition of apoptotic stimuli. Viability and cell number are determined by trypan blue exclusion from three random fields of greater than 200 cells/field. All experiments are performed a minimum of three times.(Only for Reference)
細胞系： WEHI-231 cells
- 動物實驗： 動物模型：C57BL/6 mice
- 溶解度： DMSO: 25 mg/mL
Ethanol: 100 mg/mL (194.75 mM)
（ < 1 mg l refers to the product slightly soluble or insoluble )="">
- 保存條件： -20℃
- 配置溶液濃度參考：
1mg 5mg 10mg 1 mM 1.947 ml 9.737 ml 19.475 ml 5 mM 0.389 ml 1.947 ml 3.895 ml 10 mM 0.195 ml 0.974 ml 1.947 ml 50 mM 0.039 ml 0.195 ml 0.389 ml
- 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的性，僅供客戶參考交流研究之用。
